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Drug-Target Interaction

Drug

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PubChem ID:1893
Structure:
Synonyms:
1H-Indazole, 7-nitro-
2942-42-9
5-23-06-00189 (Beilstein Handbook Reference)
7-NI
7-Nitro-1H-indazole
7-nitro-indazole
7-Nitroindazole
7-Nitroisoindazole
7NI
AC-3118
AC1L1CGU
AC1Q1X38
AC1Q1Y3U
AIDS-020326
AIDS020326
AKOS001740210
BRD-K04430056-001-02-9
BRN 0006809
BSPBio_003580
C080122
C7H5N3O2
CCG-101113
CCG-39508
CCRIS 3309
CHEBI:511442
CHEMBL247378
CPD000058266
DB02207
EINECS 220-934-4
EU-0100839
HMS2052E13
HMS2235A13
HMS3262H19
HSCI1_000055
I14-8893
IN1197
Jsp005597
KBio3_002963
Lopac-N-7778
Lopac0_000839
LS-81536
MLS000028452
MLS001074098
MolPort-000-141-465
N 7778
N0827
N7778_SIGMA
NCGC00015750-01
NCGC00015750-02
NCGC00015750-03
NCGC00015750-04
NCGC00015750-05
NCGC00015750-06
NCGC00015750-07
NCGC00015750-08
NCGC00015750-09
NCGC00015750-10
NCGC00021621-02
NCGC00021621-04
NCGC00021621-05
NCGC00021621-06
NCGC00021621-07
NCIOpen2_000477
NSC 72843
NSC72843
PQCAUHUKTBHUSA-UHFFFAOYSA-
SAM001246908
SBB002552
SMR000058266
SPBio_001730
SPECTRUM1505342
Spectrum2_001715
Spectrum3_001980
ST5405219
STK776289
Tocris-0602
ZERO/005551
ZINC13454194

Target

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Uniprot ID:Q62710_RAT
Synonyms:
Nitric oxide synthase
EC-Numbers:-
Organism:Rat
Rattus norvegicus
PDB IDs:-

Binding Affinities:

Ki: Kd:Ic 50:Ec50/Ic50:
----

References:

9176324
Macula densa stimulation of renin is reversed by selective inhibition of neuronal nitric oxide synthase.. W H Beierwaltes (1997) The American journal of physiology display abstract
The neuronal isoform of nitric oxide synthase (nNOS) exists in the renal cortex predominantly in the macula densa, suggesting that nitric oxide (NO) derived from the macula densa plays a role in feedback regulation of renin in response to altered sodium metabolism. To determine if nNOS is a critical component in renin stimulation induced by dietary sodium restriction, rats received either normal sodium or a sodium-restricted diet (0.03%) for 7 days and subsequently were or were not treated with the selective inhibitor of nNOS 7-nitroindazole (7-NI) either acutely (50 mg/kg body wt ip) on the final day or chronically (20 mg/kg body wt ip 2 x/day) over the final 5 days. On the last day, rats were anesthetized with Inactin and fitted with arterial and renal venous catheters to collect blood and monitor blood pressure (BP) and a flow probe to measure renal blood flow (RBF). BP (105 vs. 108 mmHg) was similar in normal and low-sodium dietary groups, respectively, whereas RBF tended to be higher in the sodium-restricted group (6.5 +/- 0.3 vs. 7.6 +/- 0.4 ml.min-1.g kidney wt-1). Both renal venous renin (RR) and renin secretion rate (RSR) were elevated approximately fourfold by sodium restriction [RR = 5.8 +/- 0.8 vs. 20.5 +/- 2.7 ng angiotensin (ANG) I.ml-1.h-1; P < 0.001; RSR = 3.0 +/- 0.9 vs. 13.1 +/- 4.1 ng ANG I.h-1.min-1; P < 0.025]. Acute 7-NI did not change BP, RR, or RSR, but reduced RBF in sodium-restricted rats by 8% (P < 0.05). Chronic 7-NI had no effect on renin in rats on a normal diet, but reduced RR by one-half in the sodium-restricted group (to 9.9 +/- 1.6 ng ANG I-ml-1.h-1; P < 0.001) and reduced RSR to normal (diet) levels (to 3.9 +/- 1.4 ng ANG I.h-1.min-1; P < 0.05). Although selective NOS inhibition by 7-NI did not affect BP, RBF, or renin in control rats on a normal diet, chronic 7-NI reversed the stimulation of renin induced by dietary sodium restriction. These data suggest that nNOS-derived NO plays an important role in the macula densa during feedback stimulation of renin induced by dietary sodium restriction.